CD34isawell-knownMarkerforprimitiveandbonemarrow-derivedProgenitorcells,especiallyforhematopoieticandendothelialprogenitors.ZenbiooffersCD34+progenitorcellsinaphenotypicallyundifferentiatedstate.TheprogenitorcellsarederivedfromcordbloodofhealthydonorsandproducedattheZenBiofacility.Progenitorcellscontaintwomaincellularsubpopulations,hematopoieticandendothelialprogenitorcells.CD34+progenitorcellsaresuitableforaseriesofstudiesfordirecteddifferentiationintomorecommittedtypesofbloodcellsandendotheliallineages.Immediatelyafterisolation,thefreshlypreparedCD34+progenitorcellsarecryopreservedusingaserum-freefreezingmedium.
SamplesfromeachdonoraretestedviaPCRtoconfirmnon-reactivity.
CordbloodCD133+cellsareisolatedusingdirectpositiveimmunomagneticselectionforCD133+cells.ThemajorityofCD133+cellsalsoexpressCD34.CD133isaglycoproteinwithsub-cellularlocalizationinplasmamembraneprotrusions.Inhumans,thisproteinwasoriginallyidentifiedasanantigenicmarkerexpressedonhematopoieticstemcells.Inaddition,thesecellsarehighlyproliferativeprimitivehematopoieticprogenitors.Theycangiverisetomatureterminallydifferentiatedhematopoieticcellsbymeansofdirecteddifferentiation.
ZenBiooffersCD133cellsinaphenotypicallyundifferentiatedstate.ThecellsarederivedfromfreshcordbloodofhealthydonorsandproducedattheZenBiofacility.Immediatelyafterisolation,thefreshlypreparedCD133cellsarecryopreservedusingaserum-basedcryopreservationmedium.Eachvialcontains100,000cellspervial.
