ZenbiooffersanormalhumanRenalProximalConvolutedTubuleEpithelialcellsystem.Renalproximalconvolutedtubuleepithelialcells(RPCTs)areanessentialresearchtoolforunderstandingavarietyofBIOLOGicalprocessesinthekidneys.Theycanalsobeutilizedtodeterminerenaltoxicityofcompoundsaswellastostudyunderlyingcausesandfundamentalaspectsofhumankidneydiseases.

TheseprimaryRPCTsarecollectedfromunfractionatedkidneycellpreparationsfromnormal,non-diseasedhumankidneytissues.ThecellscanbeculturedandexpandedinZenBio"suniquerenalepithelialcellculturemediaformulationsdeliveringarobustrenalcellcultureforuseinexperimentsandscreeningassays.

ZenBioprovidesRPCTscryopreservedat500,000cells/vialalongwith50mlofRPCTplatingmedia(cat#RPCT-1)foreachvialtoestablishinitialculture.PurityofeachlotisverifiedusingmorphologyandCD13flowcytometry–ZenBioRPCTsarequalifiedat>80%CD13+uponQCrelease.

EachlotofprimaryrenalcellsaretestedandverifiednegativeforHIV-1,HIV-2,HTLVI&II,HepB&C,SyphillisandCMV.

Customdonorspecificationsareavailableuponrequestandanumberofdifferentplateandflaskformatscanbecustomizedtomeetyourparticularresearchprojectneeds.

Morphology:24-48hoursafterplatingthecellsshouldappearascrescentshapedcells.

RenalProximalConvolutedTubuleEpithelialCells(RPCTs)

AlkalinePhosphataseStainingofRPCTs