SmoothMuscleCells-Theoutersmoothmusclelayerisdissectedawayfromtheinnerurothelialcelllayerofthebladderpriortoexplantculturingtoisolatethesmoothmusclecells.Smoothmusclecellsarefoundinlymphaticvessels,thewallsofbloodvessels,thebladder,theuterus,andotherareasofthebody.Theprimaryfunctionofsmoothmusclesistocreatecontractionsinthepresenceofanexternalstimulus.Thisstimulicandifferindifferentareasofthebodytogenerateindividualeffects.ATPhydrolysisprovidesenergyforsmoothmusclecontractions.Theyarecreatedbyaslidingmotionbetweenactinfilamentsandmyosin.ThesecellsexpressthesmoothmusclecellMarkerssmoothmusclealpha-actinandcalponin,andarefunctionalasdemonstratedbyacellcontractionassay.Zen-bioofferssmoothmusclecellsproducedatZenBio"sfacilityfromnormalhumantissues.Eachvialcontains500,000viablecells.
EachlotsistestedbyflowcytometryforthesmoothmusclemarkersCalponinandsmoothmusclealpha-actin.AdditionallycellshavetestednegativeforHIV-1,HIV-2,HTLV-1,HTLV-2,HepatitisB,HepatitisCandmycoplasma.
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