HUVECareisolatedfromtheveinoftheumbilicalcordandarecommonlyusedforphysiologicalandpharmacologicalinvestigations,suchasmacromoleculetransport,bloodcoagulation,angiogenesis,andfibrinolysis.Humanumbilicalveinendothelialcells(HUVECs)haveplayedamajorroleasamodelsystemforthestudyoftheregulationofendothelialcellfunctionandtheroleoftheendotheliumintheresponseofthebloodvesselwalltostretch,shearforces,andthedevelopmentofatheroscleroticplaques.
Eachhumanumbilicalcordveinisindividuallyprocessed(orpooledformixedproducts)toisolateendothelialcellsthroughCollagenasedigestionandculture.FrozenHUVECproductsarecryopreservedattheendoftheprimaryculture.HUVECareguaranteedtoreach15populationdoublingswhenculturedwithZenbio"srecommendedmedium.
SamplesfromeachdonoraretestedviaPCRtoconfirmnon-reactivity.
