Celluliteisatermappliedtoaskinconditionassociatedwithlocalizedfatdepositsthatpresentaslumpsanddimples.Fibrousstrandscalledconnectivetissue,whichseparatestheskinfromtheunderlyingfattytissueformseparatecompartmentsundertheskinthatcontainfatcells.Althoughtheetiologyofcelluliteispoorlyunderstood,themainfactorcontributingtocelluliteappearstobelocalaccumulationoffatinaregionalcompartment.

ADIpocytes(fatcells)aretheprinciplecellsimplicatedinfatstoragebyadiposetissue.Ithasbeenproposedthattheanatomicalstructureofsubcutaneousadiposetissueisamajorcontributortotheappearanceofcellulite.Whenthesefatcellsincreaseinsizetheskincompartmentbulgeswhichformscellulite.Thesefatcellscontaintriglycerides;whichmustbebrokendownbeforefatcellscanbereducedinsize.Themoretriglyceridethatisbrokendown,thesmallerthefatcellsundertheskinbecomeleavingtheskinappearingsmoother(lesscellulite).Increasedlipolysisorfatreductionofthesubcutaneousadipose(fatundertheskin)meansmoretriglycerideisbrokendownwhichleadstosmallerfatcellsandareductionofthecelluliteappearance.

Thiskitprovidesatooltostudychemicalcompoundsthatmayinfluencelipolysisinculturedhumanadipocytes.Thiskitspecificallymeasuresboththenon-esterifiedfattyacids(freefattyacids)andtheglycerolreleasedbythebreakdownoftriglyceride.

Representativedatasetobtainedfortwoconcentrationsofcustomerextractusinganti-cellulitecompoundscreeningservice;PC=positivecontrol(1uMIsoproterenol),VC=(vehiclecontrol)